Anti Lamin A C Antibody

Performed under reducing conditions.

Anti lamin a c antibody.

Western blot analysis of extracts of nci h293 cells using anti lamin a c antibody a0464. Anti lamin a lamin c antibody 131c3 nuclear envelope marker ab8984 at 1 100 dilution human fibroblast cell lysate at 15 µl developed using the ecl technique. The anti lamin a c antibody reveals strong nuclear lamina staining while anti lamp1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Lamin a and c are detected.

Lamin a c antibody 636 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. As a result this antibody recognizes lamin a but not lamin c. Lamins a and c are alternatively spliced versions of the lmna gene. This antibody has been reported by an independent laboratory to detect lamin a c in human endothelial cells.

Species predicted to react based on 100 sequence homology. The expected protein mass is 74 1 kda but there are 6 reported isoforms. See machiels et al 1997. Anti lamin a c antibody e 1 is a mouse monoclonal igg 1 kappa light chain lamin a c antibody provided at 200 µg ml.

Lamin a c antibodies. Lamin a c antibody detects endogenous levels of total full length lamin a and lamin c 70 kda as well as the small fragment of lamin a and lamin c resulting from cleavage at aspartic acid 230 28 kda. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. Reacts against lamin a exclusively the antibody was raised against the carboxy terminus of 98 amino acids present in lamin a and absent from lamin c.

Goat anti rabbit igg h l hrp as014 at 1 10 000 dilution. Application anti lamin a c antibody clone 14 detects level of lamin a c has been published validated for use in ic wb. The protein may also be known as lamin c dhe lamin a cdcd1 cddc and cmd1a. Specific for an epitope mapping between amino acids 2 29 at the n terminus of lamin a c of human origin.

Anti lamin a c antibodies are available from several suppliers. 70 kda ab8984 was used at a 1 100 dilution against human fibroblast lysate. Since both dna blue and lamin a c red are associated with the nuclear compartment this region appears crimson in this image. Raised against porcine lamin preparation.

These proteins are found in many different cell types in three different forms a b and c.