Anti Lamin Antibody What Is It

Performed under reducing conditions.

Anti lamin antibody what is it.

Anti lamin antibody products lamin is a reported alias of the human protein lamin a c encoded by the gene lmna. The full protein is reported to be 664 amino acid residues in length. Anti lamin antibodies are available from several suppliers. Anti lamin a antibody c 3 is recommended for detection of lamin a of mouse rat and human origin by wb ip if and elisa.

Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract. This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells. Fisher pa journal of cell science 108 pt 9. Analogies with vertebrate a type lamins.

Goat anti rabbit igg h l hrp preadsorbed at 1 5000 dilution developed using the ecl technique. Lamin a c antibody detects endogenous levels of total full length lamin a and lamin c 70 kda as well as the small fragment of lamin a and lamin c resulting from cleavage at aspartic acid 230 28 kda. Recombinant human lamin a protein at 0 1 µg lane 2. Specific for an epitope mapping between amino acids 580 601 near the c terminus of lamin a of human origin.

Interphase phosphorylation of the drosophila nuclear lamin. Recombinant human lamin a protein at 0 01 µg secondary all lanes. Anti lamin a antibody ab26300 at 1 µg ml lane 1. Weber k journal of cell science 108 pt 10.

That is cell receptor binding is markedly hindered. Site mapping using a monoclonal antibody. Lamins a and c are alternatively spliced versions of the lmna gene. Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx.

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. Anti lamin a c antibody e 1 is a mouse monoclonal igg 1 kappa light chain lamin a c antibody provided at 200 µg ml specific for an epitope mapping between amino acids 2 29 at the n terminus of lamin a c of human origin. Lamin a antibody c 3 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. Expression of drosophila lamin c is developmentally regulated.

Lamin a c antibodies lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. However based on bioassays it appears that the mix of antibodies in this purified polyclonal anti serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited. Species predicted to react based on 100 sequence homology.